|JOURNAL OF MEDICINE |
AND BIOMEDICAL SCIENCES
JOURNAL OF MEDICINE AND BIOMEDICAL SCIENCES (JMBS)
ISSN: 2078-0273 | Next issue: December 30, 2013
The Journal of Medicine and Biomedical Sciences is published 2 times per year in May and December. Journal publishes original papers in medicine and biomedical in general, but giving a preference to those in the areas of medicine and biomedical represented by the editorial board. All submitted papers are considered subject to the undersanding that they have not been published and are not being considered for publication elsewhere. To be publishable, papers must treat new research, be well written, and be of interest to a significant segment of the science community. There is no publication fee. But, subscribe to receive 1 print copy (see example) of the journal is compulsory for authors. Please, see Print Subscription Price List for 2014.
|December, 2013 | Vol. 4. No 2.|
Abstract: Background and Objectives: Numerous fast, sensitive and accurate microscopic methods have been developed in recent years in which Quantitative Buffy Coat (QBC) method came out as a gold standard with the advantages of high sensitivity, time saving, easy training, adaptability and simplicity of the set-up but it is very expensive and the materials are not easily obtainable. This technique was designed to quantitate malaria parasites as much as possible using the same time frame with QBC, with easily accessible materials used in the traditional methods but with simple modifications to achieve desired and equitable effects. It is very cheap, affordable and adaptable to routine diagnosis, especially in a resource-poor setting, thereby conforming to WHO standards. Results: A total of 300 blood samples were examined by QBC as well as this modified smear method. Malaria parasite was detected in 249(83%) samples using the QBC and 248(82.7%) samples with this modified smear method. All samples diagnosed as negative for malaria by QBC were equally negative by the modified smear technique. Species of malarial parasites encountered by smear examination were plasmodium falciparum (98%) while mixed infections (falciparum and vivax malaria) (2%). However, specie identification was not possible with QBC method. There was no significant difference in the time interval of analysis for QBC and modified smear methods (8.5±1.2mins, 9.4±1.5mins, respectively). Conclusion: this method (hereby referred to as Ajayi’s technique) has proven to be a reliable, adaptable, sensitive and cost-effective method for the diagnosis of malaria and possibly other haemo-parasites. Its accuracy level has also been enhanced.
Keywords: Malaria parasites, quantitative detection, enhanced accuracy, cost-effectiveness
Cite this article:
Olutayo I. Ajayi. A simple and quantitative method for the detection of malaria parasites in blood – Ajayi’s technique. Journal of Medicine and Biomedical Sciences 2013; 4(2), 5-6.
2. Selda Uzun, Omer Sayli, Yasar Tatar, Nusret Ramazonoglu, Birol Cotuk
EMG evaluation of fatigue during isometric contractions in female rowers
Abstract: Relatively few studies were carried out to quantify muscle fatigue resulting from isometric contractions in elite female athletes. The purpose of this study was to evaluate fatigue profile of 30-seconds duration isometric contractions using surface electromyography (EMG) in female elite rowers. The subjects were eleven healthy female athletes between 16-24 years of age. The Vastus Lateralis (VL) muscle of the dominant leg was chosen for the EMG recordings. Experimental set up was designed in accordance to the rowing sport. While the hip joints for all the subjects were brought to a 100o flexion, the knee joints were brought to a 90o flexion. The range of the knee joint angle (15o) during the movement was set from 90 to 105o for each subject. The maximal value of MVC was used as the reference value to determine a load of 75% of MVC for each subject. The median frequency (MDF) and the mean frequency (MNF) of the power spectrum and Root Mean Square (RMS) were computed for the surface EMG signal. Least squares line fittings were computed for time series of each parameter to find initial and final values of MDF, MNF and RMS parameters. These values were compared by Wilcoxon test. Significant decreases were found between initial and final values for both MDF and MNF (p<0.01). Also, significant decrease (p<0.01) was found between initial and final RMS. The shift of the EMG power spectrum to lower frequency content has been reported in the literature accompanied by an increase of RMS values. As only few studies have been carried out with women subjects, our RMS results may indicate a special compensatory strategy of muscle load sharing or differences in muscle fiber content in these female rowers.
Keywords: Electromyography, Female, Athletes, Rowers, Fatigue, Isometric
Cite this article:
S. Uzun, O. Sayli, Y. Tatar, N. Ramazonoglu, B. Cotuk. EMG evaluation of fatigue during isometric contractions in female rowers. Journal of Medicine and Biomedical Sciences 2013; 4(2), 7-12.
3. Jwan Ibrahim Jawzaly, Khoshawi Mussa Khalil
Assessment of glucose and bilirubin levels among premature newborns in maternity teaching hospitals
Abstract: Premature newborns are at risk of abnormalities in glucose homeostasis and development of hyperbilirubinemia. The aim of the study was to identify glucose and bilirubin levels and determine their relationship with prematurity. This descriptive study included non probability convenience samples of 206 premature newborns. Information collected from premature newborn birth documents comprised ̵ ; birth weight and length measurements, blood glucose levels, total serum bilirubin and packed cell volume (PCV) percentage, maternal age and obstetric properties were also collected. Most of mothers were in the age group of (15 ̵ 24) years, with no history of abortion and one child . Most newborns had gestational age less than 32 weeks . Mean of premature newborns weight was 1847.9± 629.5 g and majority were in the range of (1500 ̵ 1999 g). Mean length of premature newborn was 37.5± 4.6 cm and majority were (35 and 40 cm) in length. Mean total bilirubin was 6.07 ± 5.7 mg/dl . Mean PCV percentage was 59.88% ± 9.24. Mean random blood sugar levels was 79.7 ± 47.04 mg/dl. A significant negative correlation was found between random blood sugar and body length and PCV, whereas a negative correlation was observed with premature newborns weight. Bilirubin also showed a significant negative correlation with PCV. Prematurity caused by young maternal age was reflected in growth and glucose tolerance of newborns, consequently affecting their hemolysis status and bilirubin production.
Keywords: Glucose; Bilirubin; Heamolysis status; Premature newborns
Cite this article:
Jwan I. Jawzaly, Khoshawi M. Khalil. Assessment of glucose and bilirubin levels among premature newborns in maternity teaching hospitals. Journal of Medicine and Biomedical Sciences 2013; 4(2), 13-19.
4. Yosef Orabi, Mohamed Fath EL-Bab
Electrical activity and nerve conduction in gymnastic players
Abstract: The peripheral nerves of the upper extremity are exposed to acute and chronic mechanical injuries in gymnasts, because of the high repetition of motions, high muscular forces and extreme joints positions. The main aim of this study was to assess and describe nerve conduction velocities in Median and Ulnar nerves in gymnasts. The study is a prospective descriptive study of age matched 5 males gymnasts with mean age of (19.40± 0.55) from Taibah University in AL-Madinah AL-Munawarah in Kingdom of Saudi Arabia in the study year of 2012/2013 compared with 5 healthy male non-active controls, aged (20.00± 0.71) years. Written informed consent was obtained from each volunteer after the protocol was approved by the Taibah University local ethics committee. Anthropometric measurements (height, weight, limb length, and perimeters of arm and forearm) were determined for each subject, and range of motion assessment and radiographic examination carried out. Standard Nerve Conduction Velocity (NCV) techniques using constant measured distances were applied to evaluate the Median and Ulnar nerves in the dominant and non-dominant limb of each individual. Data were calculated and compared by using SPSS version 13.0. We found that the sensory and motor conduction velocities of the Median and the Ulnar nerves were significantly different in gymnastic players compared with normal subjects. There were no statistical differences in the latencies, amplitudes, or the conduction velocities of the F waves recording of both gymnasts Ulnar and Median motor when compared with the normal controls. In conclusion the gymnasts show an affected nerve conduction velocity compared to the normal controls, further NCV and electromyography investigations are needed for the gymnasts.
Keywords: Gymnastics; Wrist Injuries; Grip Lock
Cite this article:
Y. Orabi, M. Fath EL-Bab. Electrical activity and nerve conduction in gymnastic players. Journal of Medicine and Biomedical Sciences 2013; 4(2), 20-27.
5. Sungai Mazando, M. Zimba, C. Zimudzi, S. Dumbura, M. Gundidza
Proposed contribution of Rv1819c an efflux pump (ABC protein) from Mycobacterium tuberculosis to drug efflux in Corynebacterium glutamicum
Abstract: Multidrug-resistant tuberculosis (MDR-TB) is among the most worrisome aspects of the pandemic of antibiotic resistance because TB patients that fail treatment have a high risk of death. Active efflux pumps play an important role in bacterial resistance to antibiotics. Rv1819c, a putative efflux pump protein gene from Mycobacterium tuberculosis was cloned and transformed into Corynebacterium glutamicum. Susceptibility to ciprofloxacin with and without efflux pump inhibitors (CCCP, reserpine and verapamil) was determined using the agar disc diffusion method. Ciprofloxacin uptake/efflux experiments were performed in C. glutamicum expressing Rv1819c. Cells expressing the Rv1819c insert had a higher efflux activity than cells that were expressing the plasmid without the gene insert. The cells expressing Rv1819c insert had higher MICs (0.64 µg/disc) compared to cells that were not expressing the Rv1819c gene (less than 0.08 µg/disc). There was evidence of energy-dependent efflux of ciprofloxacin from C. glutamicum expressing Rv1819c as shown by the increase in the intracellular concentration of ciprofloxacin when the cells were incubated with efflux pump inhibitors reserpine, verapamil and CCCP. Thus, Rv1819c efflux pump of M. tuberculosis may contribute to intrinsic ciprofloxacin efflux and, hence, drug resistance by actively extruding the drug from the cell via ATP- mediated drug extrusion.
Keywords: Mycobacterium tuberculosis, Rv1819c, antibiotics, ABC efflux pump, Corynebacterium glutamicum, drug resistance, efflux pump inhibitors
Cite this article:
S. Mazando, M. Zimba, C. Zimudzi, S. Dumbura, M. Gundidza. Proposed contribution of Rv1819c an efflux pump (ABC protein) from Mycobacterium tuberculosis to drug efflux in Corynebacterium glutamicum. Journal of Medicine and Biomedical Sciences 2013; 4(2), 28-36.
Abstract: The student nurses socialization in to the profession is dependent upon acceptance and approval by staff nurses who have the greatest influence on the development of the student nurse's profession role. The study aimed to identify knowledge and attitudes of nursing students. The study began in 1/7/ 2011, to 3/3/ 2012. the Questionnaire was designed for data collection and viewed to 10 experts in nursing field for validity and the questionnaire was consisted of two main parts included sociodemographic characteristics and questions related to nursing. The results of the study indicated that the majority of them have knowledge about nursing, they have positive attitudes but most of them not satisfied with nursing services in hospitals and there is not significant effect of age and sex on their attitude and knowledge. The study recommended There must be cooperation between ministry of health and ministry of higher education regarding improving training and nursing services in hospitals. Encourage the female students to involve in this field to increase the quality of nursing services.
Keywords: Knowledge, attitude, nursing profession, nursing students
Cite this article:
Kareem F. Aziz. Knowledge and attitudes of nursing students toward nursing as a profession in medical technical institute in Erbil- Iraq. Journal of Medicine and Biomedical Sciences 2013; 4(2), 37-41.